Three-Dimensional Bioprinting of Ovine Aortic Valve Endothelial and Interstitial Cells for the Development of Multicellular Tissue Engineered Tissue Constructs

To investigate the pathogenic mechanisms of calcified aortic valve disease (CAVD), it is necessary to develop a new three-dimensional model that contains valvular interstitial cells (VIC) and endothelial (VEC). For this purpose, ovine valves were processed isolate VIC VEC dissolved in an alginate/gelatin hydrogel. A 3D-bioprinter (3D-Bioplotter® Developer Series, EnvisionTec, Gladbeck, Germany) was used print cell-laden tissue constructs containing which cultured for up 21 days. The 3D-architecture, composition culture medium, hydrogels modified, cell viability assessed. medium directly affected multicellular constructs. Co-culture with mixture 70% 30% components reached best tested about 60% more living compared pure (p = 0.02). retained comparable after days 0.90) different 3D-architectures, including “sandwich” “tube” design. Good long-term confirmed even thick multilayer 3D-bioprinting successful technique design mimic structure native research applications pathologies.